pcdna3 1 cmv chrna7 (Addgene inc)
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pcdna3 1 cmv chrna7
Pcdna3 1 Cmv Chrna7, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+cmv+chrna7/pcDNA3%2E1-CHRNA7+(Plasmid+%2362276)/pm41922647-18-68-78
Average 93 stars, based on 15 article reviews
Pcdna3 1 Cmv Chrna7, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+cmv+chrna7/pcDNA3%2E1-CHRNA7+(Plasmid+%2362276)/pm41922647-18-68-78
Average 93 stars, based on 15 article reviews
pcdna3 1 cmv chrna7 - by Bioz Stars,
2026-09
93/100 stars
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Related Articles
Plasmid Preparation:Article Title: AAV.PHP.eB-based strategies for precise modulation of α7 nicotinic acetylcholine receptor in neurons and astrocytes in the adult mouse brain. Article Snippet: .. Plasmid design and viral vector assembly for CHRNA7 overexpression or silencing To generate plasmids capable of overexpressing CHRNA7 selectively in neurons and astrocytes, the CHRNA7 coding sequence from plasmid Addgene #62276 was amplified by PCR and inserted into two in-house pAAV2 viral backbones, containing the hSyn or GFAP promoters as well as mRuby2 gene reporter, using Gibson Assembly (Gibson Assembly Master Mix, New England Biolabs, Ipswich, MA, USA). Over Expression:Article Title: AAV.PHP.eB-based strategies for precise modulation of α7 nicotinic acetylcholine receptor in neurons and astrocytes in the adult mouse brain. Article Snippet: .. Plasmid design and viral vector assembly for CHRNA7 overexpression or silencing To generate plasmids capable of overexpressing CHRNA7 selectively in neurons and astrocytes, the CHRNA7 coding sequence from plasmid Addgene #62276 was amplified by PCR and inserted into two in-house pAAV2 viral backbones, containing the hSyn or GFAP promoters as well as mRuby2 gene reporter, using Gibson Assembly (Gibson Assembly Master Mix, New England Biolabs, Ipswich, MA, USA). Sequencing:Article Title: AAV.PHP.eB-based strategies for precise modulation of α7 nicotinic acetylcholine receptor in neurons and astrocytes in the adult mouse brain. Article Snippet: .. Plasmid design and viral vector assembly for CHRNA7 overexpression or silencing To generate plasmids capable of overexpressing CHRNA7 selectively in neurons and astrocytes, the CHRNA7 coding sequence from plasmid Addgene #62276 was amplified by PCR and inserted into two in-house pAAV2 viral backbones, containing the hSyn or GFAP promoters as well as mRuby2 gene reporter, using Gibson Assembly (Gibson Assembly Master Mix, New England Biolabs, Ipswich, MA, USA). Amplification:Article Title: AAV.PHP.eB-based strategies for precise modulation of α7 nicotinic acetylcholine receptor in neurons and astrocytes in the adult mouse brain. Article Snippet: .. Plasmid design and viral vector assembly for CHRNA7 overexpression or silencing To generate plasmids capable of overexpressing CHRNA7 selectively in neurons and astrocytes, the CHRNA7 coding sequence from plasmid Addgene #62276 was amplified by PCR and inserted into two in-house pAAV2 viral backbones, containing the hSyn or GFAP promoters as well as mRuby2 gene reporter, using Gibson Assembly (Gibson Assembly Master Mix, New England Biolabs, Ipswich, MA, USA). Polymerase Chain Reaction:Article Title: AAV.PHP.eB-based strategies for precise modulation of α7 nicotinic acetylcholine receptor in neurons and astrocytes in the adult mouse brain. Article Snippet: .. Plasmid design and viral vector assembly for CHRNA7 overexpression or silencing To generate plasmids capable of overexpressing CHRNA7 selectively in neurons and astrocytes, the CHRNA7 coding sequence from plasmid Addgene #62276 was amplified by PCR and inserted into two in-house pAAV2 viral backbones, containing the hSyn or GFAP promoters as well as mRuby2 gene reporter, using Gibson Assembly (Gibson Assembly Master Mix, New England Biolabs, Ipswich, MA, USA). |